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genechip fluidics station 450  (Thermo Fisher)


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    Structured Review

    Thermo Fisher genechip fluidics station 450
    Genechip Fluidics Station 450, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/genechip+fluidic+station+450/Streptavidin/pm41479162-45-10-14
    Average 99 stars, based on 1 article reviews
    genechip fluidics station 450 - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Staining:

    Article Title: Microarray-Based Sketches of the HERV Transcriptome Landscape
    Article Snippet: .. Washing and staining were carried out according to the protocol supplied by the manufacturer, using a fluidic station (GeneChip fluidic station 450, Affymetrix). .. The arrays were scanned using a fluorometric scanner (GeneChip scanner GS 3000, Affymetrix).

    Article Title: Frequent copy number variants in a cohort of Mexican-Mestizo individuals
    Article Snippet: .. Arrays were stained and washed in the Affymetrix GeneChip Fluidic Station 450 and scanned using an Affymetrix GeneChip Scanner 3000 7G (Affymetrix, Santa Clara, CA, USA). ..

    Article Title: Distinct Signature of Altered Homeostasis in Aging Rod Photoreceptors: Implications for Retinal Diseases
    Article Snippet: FL-Ovation cDNA Biotin Module V2 (NuGEN Technologies) was used to fragment and biotin label the amplified cDNA and for hybridization of the labeled target onto a GeneChip Mouse Genome 430 2.0 Array (Affymetrix, Santa Clara, CA) with 45,000 probe sets for 34,000 well-characterized mouse genes. .. Washing and staining of the GeneChips was performed using the EukGE-WS2v4_450 protocol on the GeneChip Fluidic Station 450 (Affymetrix). .. GeneChip scanning was performed on the GCS3000-7G Scanner (Affymetrix).

    Article Title: Effect of Protein Kinase C delta (PKC-δ) Inhibition on the Transcriptome of Normal and Systemic Sclerosis Human Dermal Fibroblasts In Vitro
    Article Snippet: The labeled cRNA was fragmented by heat and ion-mediated hydrolysis and was hybridized to the Human Genome HU133A oligonucleotide array GeneChip (Affymetrix) which contains ∼500,000 spots comprised of 22,283 different probe sets representing 14,397 unique genes. .. The arrays were washed and stained using a GeneChip Fluidic Station 450 (Affymetrix), and hybridization signals were amplified utilizing antibody amplification with goat IgG (Sigma-Aldrich, Saint Louis, MO) and anti-streptavidin biotinylated antibody (Vector Laboratories, Burlingame, CA) and then were scanned using an Affymetrix GeneChip Scanner 3000, using GeneChip Operating Software (GCOS) version 3.0. .. Background correction and normalization were done using a Robust Multichip Average (RMA) with Genespring V 10.0 software (Agilent Technologies, Santa Clara, CA).

    Article Title: Hypoxia and Human Genome Stability: Downregulation of BRCA2 Expression in Breast Cancer Cell Lines
    Article Snippet: Fragmented cRNA was hybridized using a human oligonucleotide array U133 Plus 2.0 (Genechip Affymetrix, Santa Clara, CA, USA). .. Washing and staining were performed through Affymetrix GeneChip Fluidic Station 450. .. Probe arrays were scanned using Affymetrix GeneChipScanner3000 G7enabled for high-resolution scanning.

    Hybridization:

    Article Title: R -Roscovitine (Seliciclib) prevents DNA damage-induced cyclin A1 upregulation and hinders non-homologous end-joining (NHEJ) DNA repair
    Article Snippet: Labelled cRNA was isolated by using the RNeasy Mini Kit column (QIAGEN). .. After hybridisation, chips were processed by using the Affymetrix GeneChip Fluidic Station 450 (protocol EukGE-WS2v5_450). .. The quality of total RNA, cDNA synthesis, cRNA amplification and cRNA fragmentation was monitored by capillary electrophoresis (Bioanalizer 2100, Agilent Technologies).

    Article Title: Gene expression profile of androgen modulated genes in the murine fetal developing lung
    Article Snippet: Fifteen micrograms of fragmented cRNA was hybridized for 16 h at 45°C with constant rotation, using a mouse oligonucleotide array MOE430 2.0 (Genechip, Affymetrix, Santa Clara, CA). .. After hybridization, chips were processed by using the Affymetrix GeneChip Fluidic Station 450 (protocol EukGE-WS2v5_450). .. Staining was made with streptavidin-conjugated phycoerythrin (SAPE; Molecular Probes), followed by amplification with a biotinylated anti-streptavidin antibody (Vector Laboratories), and by a second round of SAPE.

    Article Title: Effect of Protein Kinase C delta (PKC-δ) Inhibition on the Transcriptome of Normal and Systemic Sclerosis Human Dermal Fibroblasts In Vitro
    Article Snippet: The labeled cRNA was fragmented by heat and ion-mediated hydrolysis and was hybridized to the Human Genome HU133A oligonucleotide array GeneChip (Affymetrix) which contains ∼500,000 spots comprised of 22,283 different probe sets representing 14,397 unique genes. .. The arrays were washed and stained using a GeneChip Fluidic Station 450 (Affymetrix), and hybridization signals were amplified utilizing antibody amplification with goat IgG (Sigma-Aldrich, Saint Louis, MO) and anti-streptavidin biotinylated antibody (Vector Laboratories, Burlingame, CA) and then were scanned using an Affymetrix GeneChip Scanner 3000, using GeneChip Operating Software (GCOS) version 3.0. .. Background correction and normalization were done using a Robust Multichip Average (RMA) with Genespring V 10.0 software (Agilent Technologies, Santa Clara, CA).

    Amplification:

    Article Title: Effect of Protein Kinase C delta (PKC-δ) Inhibition on the Transcriptome of Normal and Systemic Sclerosis Human Dermal Fibroblasts In Vitro
    Article Snippet: The labeled cRNA was fragmented by heat and ion-mediated hydrolysis and was hybridized to the Human Genome HU133A oligonucleotide array GeneChip (Affymetrix) which contains ∼500,000 spots comprised of 22,283 different probe sets representing 14,397 unique genes. .. The arrays were washed and stained using a GeneChip Fluidic Station 450 (Affymetrix), and hybridization signals were amplified utilizing antibody amplification with goat IgG (Sigma-Aldrich, Saint Louis, MO) and anti-streptavidin biotinylated antibody (Vector Laboratories, Burlingame, CA) and then were scanned using an Affymetrix GeneChip Scanner 3000, using GeneChip Operating Software (GCOS) version 3.0. .. Background correction and normalization were done using a Robust Multichip Average (RMA) with Genespring V 10.0 software (Agilent Technologies, Santa Clara, CA).

    Software:

    Article Title: Effect of Protein Kinase C delta (PKC-δ) Inhibition on the Transcriptome of Normal and Systemic Sclerosis Human Dermal Fibroblasts In Vitro
    Article Snippet: The labeled cRNA was fragmented by heat and ion-mediated hydrolysis and was hybridized to the Human Genome HU133A oligonucleotide array GeneChip (Affymetrix) which contains ∼500,000 spots comprised of 22,283 different probe sets representing 14,397 unique genes. .. The arrays were washed and stained using a GeneChip Fluidic Station 450 (Affymetrix), and hybridization signals were amplified utilizing antibody amplification with goat IgG (Sigma-Aldrich, Saint Louis, MO) and anti-streptavidin biotinylated antibody (Vector Laboratories, Burlingame, CA) and then were scanned using an Affymetrix GeneChip Scanner 3000, using GeneChip Operating Software (GCOS) version 3.0. .. Background correction and normalization were done using a Robust Multichip Average (RMA) with Genespring V 10.0 software (Agilent Technologies, Santa Clara, CA).



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